Journal: Cancer Cell International

Article Title: DDX10 promotes the proliferation and metastasis of colorectal cancer cells via splicing RPL35

doi: 10.1186/s12935-022-02478-1

Figure Lengend Snippet: DDX10 alternatively splices the mRNA of RPL35 then work through E2F pathway or immune response of CRC A RPL35 interacts with DDX10 through the result of Co-IP; B Overexpression of DDX10 obviously reduced the expression of RPL35 at mRNA level via q-PCR; C Overexpression of DDX10 obviously reduced the expression of RPL35 at protein level; D The expression of two mRNA isoforms of RPL35 in normal HCT116 cells and DDX10 knockdown HCT116 cells by q-PCR; E DDX10 and RPL35 are closely related to E2F1 and E2F4 from the data of LinkedOmics; F DDX10 interacts with RPL35 to regulate E2F1 and E2F4 to regulate colorectal cancer; G The relationship between DDX10 and immune cells that infiltrate the tumor tissue in COAD

Article Snippet: The primary antibodies used in our study were mouse anti-GAPDH (1:5000; Santa Cruz, USA), rabbit anti-RPL35 (1:100; ABclonal, China), rabbit anti-DDX10 (1:300; Proteintech, USA), mouse anti-FLAG (1:2,000; Sigma, USA), rabbit anti-RPL18 (1:100; ABclonal, China), rabbit anti-HNRNPU (1:100; ABclonal, China), rabbit anti-NCL (1:100; ABclonal, China), rabbit anti-PRMT5 (1:100; ABclonal, China), rabbit anti-E2F1 (1:100; ABclonal, China), rabbit anti-E2F4 (1:100; ABclonal, China), rabbit anti-E2F8 (1:100; ABclonal, China), goat anti-rabbit IgG (1:2,000; CST, USA), and goat anti-mouse IgG (1:2,000; CST, USA).

Techniques: Co-Immunoprecipitation Assay, Over Expression, Expressing

Journal: Molecular Neurobiology

Article Title: A Mutant Variant of E2F4 Triggers Multifactorial Therapeutic Effects in 5xFAD Mice

doi: 10.1007/s12035-022-02764-z

Figure Lengend Snippet: Expression of tau, E2F4 and E2F4DN-myc in the hippocampus and cerebral cortex of transgenic mice. a Western blot analysis of hippocampal extracts from 3-month-old mice from the indicated genotypes ( n = 3 females/genotype, except WT/E2F4DN: 1 male and 2 females) using antibodies against tau protein, E2F4, myc tag and Actin (as a loading control). Original western blots from this and the rest of figures can be seen in Supplementary Fig. . b Quantification of the ratios of Tau and E2F4 vs Actin in the hippocampus from the indicated genotypes. * p < 0.05 (two-way ANOVA, followed by post hoc Student’s t test). c Western blot analysis of cortical extracts from 3-month-old mice from the indicated genotypes ( n = 3 females/genotype, except WT/E2F4DN: 1 male and 2 females) using antibodies against tau protein, E2F4, myc tag, and Actin (as a loading control). d Quantification of the ratios of Tau and E2F4 vs Actin in the cerebral cortex from the indicated genotypes. ** p < 0.01 (two-way ANOVA, followed by post hoc Student’s t test)

Article Snippet: The rabbit anti-E2F4 polyclonal antibody (LS-B1532; LSBio) was used at 1:400 for proximity ligation assay (PLA) and immunohistochemistry.

Techniques: Expressing, Transgenic Assay, Western Blot, Control